Identification of E6AP/UBE3A as a ubiquitin‐protein ligase that catalyzes encephalomyocarditis virus 3C protease ubiquitylation (952.1)
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چکیده
منابع مشابه
Identification and characterization of a 3C-like protease from rabbit hemorrhagic disease virus, a calicivirus.
Expression studies conducted in vitro and in Escherichia coli led to the identification of a protease from rabbit hemorrhagic disease virus (RHDV). The gene coding for this protease was found to be located in the central part of the genome preceding the putative RNA polymerase gene. It was demonstrated that the protease specifically cuts RHDV polyprotein substrates both in cis and in trans. Sit...
متن کاملEncephalomyocarditis Virus 3C Protease relieves TANK Inhibitory Effect on TRAF6-mediated NF-kappaB Signaling through Cleavage of TANK
TRAF family member-associated NF-κB activator (TANK) is a negative regulator of canonical NF-κB signaling in the Toll-like receptor (TLR)and B-cell receptor (BLR)-mediated signaling pathways. However, functions of TANK in viral infection-mediated NF-κB activation remain unclear. Here we reported that TANK was cleaved by encephalomyocarditis virus (EMCV) 3C at the 197 and 291 glutamine residues,...
متن کاملEncephalomyocarditis virus 3C protease attenuates type I interferon production through disrupting the TANK–TBK1–IKKε–IRF3 complex
TRAF family member-associated NF-κB activator (TANK) is a scaffold protein that assembles into the interferon (IFN) regulator factor 3 (IRF3)-phosphorylating TANK-binding kinase 1 (TBK1)-(IκB) kinase ε (IKKε) complex, where it is involved in regulating phosphorylation of the IRF3 and IFN production. However, the functions of TANK in encephalomyocarditis virus (EMCV) infection-induced type I IFN...
متن کاملPolyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis.
A genomic clone of the small, round-structured virus Southampton virus (SV) was constructed from a set of overlapping PCR amplicons. Sequence analysis confirmed the absence of mutations and accurate ligation of the PCR products. The SV cDNA was cloned into a vector for in vitro production of RNA and subsequent translation by rabbit reticulocyte lysate. Two polypeptides corresponding to the N-te...
متن کاملIdentification of active-site amino acid residues in the Chiba virus 3C-like protease.
The 3C-like protease of the Chiba virus, a Norwalk-like virus, is one of the chymotrypsin-like proteases. To identify active-site amino acid residues in this protease, 37 charged amino acid residues and a putative nucleophile, Cys139, within the GDCG sequence were individually replaced with Ala in the 3BC precursor, followed by expression in Escherichia coli, where the active 3C-like protease w...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2014
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.28.1_supplement.952.1